产品中心
-
分子生物学
-
细胞生物学
-
蛋白质组学
-
蛋白凝胶纯化
-
生化试剂
-
仪器设备
-
通用实验耗材
-
色谱耗材
浏览历史
产品概况
| 货号 | EK0660 |
|---|---|
| 产品名称 | Mouse AXL ELISA Kit |
| 产品描述 | For quantitative detection of mouse AXL in , cell lysates |
| 免疫原 | NSO, H20-P443 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | AXL |
| 基因全名 | AXL receptor tyrosine kinase |
| Uniprot ID | Q00993 |
| 指标别名 | Adhesion related kinase|AI323647|Ark|AXL oncogene|AXL receptor tyrosine kinase|AXL transforming gene|AXL transforming sequence/gene|JTK11|Oncogene AXL|Tyro7|Tyrosine protein kinase receptor UFO|UFO |
| 背景资料 | Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants. |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0660-CAP | 96-well plate precoated with anti- mouse AXL antibody | 1 |
| EK0660-ST | Lyophilized recombinant mouse AXL standard | 10ng/tubex2 |
| EK0660-DA | Biotinylated anti- mouse AXL antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 53 | 280 | 992 | 48 | 266 | 1046 |
| Standard deviation | 3.86 | 14.84 | 66.46 | 3.55 | 14.09 | 81.58 |
| CV(%) | 7.3% | 5.3% | 6.7% | 7.4% | 5.3% | 7.8% |
Mouse AXL ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.063 | 0.090 | 0.135 | 0.201 | 0.345 | 0.655 | 1.155 | 2.066 |
*典型数据仅供参考。用户需要自己建立标准曲线。
暂无产品信息
暂无参考文献
暂无文件下载
COA检索
请输入批号:
.png)
