Anti-CD4 Antibody(Monoclonal, CA-4)

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分子生物学
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品名：Anti-CD4 Antibody(Monoclonal, CA-4)
品牌：博士德
货号：BM0212

货号：BM0212

应用：IHC-F, ICC, Flow Cyt

反应性：Human

克隆性：单克隆抗体

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SKU	包装规格	单位	目录价	您的价格	库存
BM0212-100ul	100ul	EA	¥1780.00	请登录	请登录
BM0212-150ul	150ul	EA	¥2580.00	请登录	请登录
BM0212-50ul	50ul	EA	¥1060.00	请登录	请登录

产品概况

货号	BM0212
产品名称	Anti-CD4 Antibody(Monoclonal, CA-4)
基因名	CD4
抗体来源	Mouse
克隆	Monoclonal (Clone: CA-4)
抗体亚型	Mouse IgG1
免疫原	CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.
内容	200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式	Ascites
浓度	200ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
研究类别	1. Committee on Human Leukocyte Differentiation Antigens, IUIS WHO Nomenclature Subcommittee : Proposed nomenclature for human leukocyte differentiation antigens. Bull. WHO 5: 809-811, 1984. Note: Alternate: Immunology Today 5: 280 only, 1984. 2. Grakoui, A.; Bromley, S. K.; Sumen, C.; Davis, M. M.; Shaw, A. S.; Allen, P. M.; Dustin, M. L. : The immunological synapse: a molecular machine controlling T cell activation. Science 285: 221-227, 1999.
Uniprot ID	CD4: P01730
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Immunohistochemistry(Frozen Section):	1:50-400
Immunocytochemistry:	1:50-400
Flow Cytometry:	1-3 μg/1x10⁶ cells
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD4 antibody (BM0212).Overlay histogram showing human PBMC cells stained with BM0212 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (BM0212,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]

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