货号 | BM0212 |
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产品名称 | Anti-CD4 Antibody(Monoclonal, CA-4) |
基因名 | CD4 |
抗体来源 | Mouse |
克隆 | Monoclonal (Clone: CA-4) |
抗体亚型 | Mouse IgG1 |
免疫原 | CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. |
内容 | 200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol. |
纯化方式 | Ascites |
浓度 | 200ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase. |
研究类别 | 1. Committee on Human Leukocyte Differentiation Antigens, IUIS WHO Nomenclature Subcommittee : Proposed nomenclature for human leukocyte differentiation antigens. Bull. WHO 5: 809-811, 1984. Note: Alternate: Immunology Today 5: 280 only, 1984. 2. Grakoui, A.; Bromley, S. K.; Sumen, C.; Davis, M. M.; Shaw, A. S.; Allen, P. M.; Dustin, M. L. : The immunological synapse: a molecular machine controlling T cell activation. Science 285: 221-227, 1999. |
Uniprot ID | CD4: P01730 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Immunohistochemistry(Frozen Section): | 1:50-400 |
Immunocytochemistry: | 1:50-400 |
Flow Cytometry: | 1-3 μg/1x106 cells |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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