产品概况
货号 | EK0673 |
---|---|
产品名称 | Mouse cathepsin D ELISA Kit |
产品描述 | For quantitative detection of mouse Cathepsin D in , cell lysates |
免疫原 | NSO, I21-L410 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | CTSD |
基因全名 | cathepsin D |
Uniprot ID | P18242 |
指标别名 | CATD_HUMAN|Cathepsin D heavy chain|Ceroid lipofuscinosis neuronal 10|CLN10|CPSD|ctsd|Lysosomal aspartyl protease |
背景资料 | Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker. |
显色时间 | 15-20分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0673-CAP | 96-well plate precoated with anti- mouse Cathepsin D antibody | 1 |
EK0673-ST | Lyophilized recombinant mouse Cathepsin D standard | 20ng/tubex2 |
EK0673-DA | Biotinylated anti- mouse Cathepsin D antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 844 | 1778 | 7768 | 898 | 1799 | 8034 |
Standard deviation | 63.3 | 99.56 | 458.31 | 76.33 | 133.12 | 514.17 |
CV(%) | 7.5% | 5.6% | 5.9% | 8.5% | 7.4% | 6.4% |
Mouse cathepsin D ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 15-20分钟)
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.246 | 0.282 | 0.329 | 0.444 | 0.608 | 1.021 | 1.350 | 1.919 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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