Mouse CD169/SIGLEC-1 ELISA Kit

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货号：EK1305

反应性：Mouse

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
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产品概况

货号	EK1305
产品名称	Mouse CD169/SIGLEC-1 ELISA Kit
产品描述	For quantitative detection of mouse CD169 in , cell lysates
免疫原	NSO, T20-R1639
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	156pg/ml-10000pg/ml
基因名称	SIGLEC1
基因全名	sialic acid binding Ig-like lectin 1, sialoadhesin
Uniprot ID	Q62230
指标别名	CD169\|CD169 antigen\|dJ1009E24.1\|DKFZp667F058\|FLJ00051\|FLJ00055\|FLJ00073\|FLJ00411\|FLJ32150\|Sialic acid binding Ig like lectin 1\|Sialic acid binding immunoglobulin like lectin 1\|Sialic acid-binding Ig-like lectin 1\|Sialoadhesin\|Siglec 1\|Siglec-1\|Siglec1\|SN\|SN_HUMAN
背景资料	SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
显色时间	20-25分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1305-CAP	96-well plate precoated with anti- mouse CD169 antibody	1
EK1305-ST	Lyophilized recombinant mouse CD169 standard	10ng/tube×2
EK1305-DA	Biotinylated anti- mouse CD169 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Mouse CD169/SIGLEC-1 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 20-25分钟)

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.011	0.186	0.334	0.544	0.973	1.571	2.031	2.394

*典型数据仅供参考。用户需要自己建立标准曲线。

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