产品概况
货号 | EK1305 |
---|---|
产品名称 | Mouse CD169/SIGLEC-1 ELISA Kit |
产品描述 | For quantitative detection of mouse CD169 in , cell lysates |
免疫原 | NSO, T20-R1639 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | SIGLEC1 |
基因全名 | sialic acid binding Ig-like lectin 1, sialoadhesin |
Uniprot ID | Q62230 |
指标别名 | CD169|CD169 antigen|dJ1009E24.1|DKFZp667F058|FLJ00051|FLJ00055|FLJ00073|FLJ00411|FLJ32150|Sialic acid binding Ig like lectin 1|Sialic acid binding immunoglobulin like lectin 1|Sialic acid-binding Ig-like lectin 1|Sialoadhesin|Siglec 1|Siglec-1|Siglec1|SN|SN_HUMAN |
背景资料 | SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses. |
显色时间 | 20-25分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1305-CAP | 96-well plate precoated with anti- mouse CD169 antibody | 1 |
EK1305-ST | Lyophilized recombinant mouse CD169 standard | 10ng/tube×2 |
EK1305-DA | Biotinylated anti- mouse CD169 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 331 | 1301 | 5540 | 351 | 1258 | 5216 |
Standard deviation | 21.18 | 84.56 | 371.18 | 26.67 | 86.8 | 432.92 |
CV(%) | 6.4% | 6.5% | 6.7% | 7.6% | 6.9% | 8.3% |
Mouse CD169/SIGLEC-1 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 20-25分钟)
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.011 | 0.186 | 0.334 | 0.544 | 0.973 | 1.571 | 2.031 | 2.394 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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