Human CD163 ELISA Kit

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货号：EK0715

反应性：Human

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK0715-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK0715
产品名称	Human CD163 ELISA Kit
产品描述	For quantitative detection of human CD163 in , serum and plasma(heparin).
免疫原	NSO, G41-S1045
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<150pg/ml
检测范围	1.56ng/ml-100ng/ml
基因名称	CD163
基因全名	CD163 molecule
Uniprot ID	Q86VB7
指标别名	CD_antigen=CD163\|CD163\|Hemoglobin scavenger receptor\|M130\|MM130\|Scavenger receptor cysteine-rich type 1 protein M130
背景资料	CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
显色时间	15-20分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0715-CAP	96-well plate precoated with anti- human CD163 antibody	1
EK0715-ST0	Lyophilized recombinant human CD163 standard	100ng/tube×2
EK0715-DA	Biotinylated anti- human CD163 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Human CD163 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 15-20分钟)

Concentration(ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.012	0.046	0.096	0.205	0.449	0.973	1.864	2.469

*典型数据仅供参考。用户需要自己建立标准曲线。

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