Mouse CCL19/MIP-3 beta ELISA Kit

货号:EK0457
反应性:Mouse
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产品概况

货号EK0457
产品名称Mouse MIP-3β ELISA Kit
产品描述For quantitative detection of mouse CCL19 in , cell lysates
免疫原E.coli, G26-S108
交叉反应There is no detectable cross-reactivity with other relevant proteins.
敏感性<10pg/ml
检测范围15.6pg/ml-1000pg/ml
基因名称CCL19
基因全名chemokine (C-C motif) ligand 19
Uniprot IDO70460
指标别名Beta chemokine exodus 3|Beta-chemokine exodus-3|C C chemokine ligand 19|C-C motif chemokine 19|CC chemokine ligand 19|CCL19|CCL19_HUMAN|Chemokine (C C motif) ligand 19|Chemokine (CC motif) ligand 19|Chemokine CC Motif Ligand 19|CK beta 11|CK beta-11|CKb11|EBI1 ligand chemokine|ELC|Epstein-Barr virus-induced molecule 1 ligand chemokine|Exodus 3|Macrophage inflammatory protein 3 beta|MGC34433|MIP 3 beta|MIP-3-beta|MIP-3B|MIP3 beta|MIP3B|OTTHUMP00000000531|SCYA19|Small inducible cytokine A19|Small-inducible cytokine A19
背景资料Chemokine(C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was mapped to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.
显色时间25-30分钟

试剂盒内容

SKU名称数量
Catalog numberDescriptionQuantity
EK0457-CAP96-well plate precoated with anti- mouse CCL19 antibody1
EK0457-STLyophilized recombinant mouse CCL19 standard10ng/tube×2
EK0457-DABiotinylated anti- mouse CCL19 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

  • 1.96孔读板仪器。
  • 2.自动洗板机.
  • 3.移液器和枪头。建议使用多通道移液器/排枪。
  • 4.Eppendorf 管。
  • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
    • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3016138427175349
Standard deviation1.28.5329.561.459.2733.5
CV(%)4%5.3%7.7%5.4%5.3%9.6%

Mouse MIP-3β ELISA Kit的典型数据示例(取自最近的一批次质检数据)

(TMB 孵育条件: 37°C, 25-30分钟)
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0110.0370.0730.1220.2700.6441.4422.317
*典型数据仅供参考。用户需要自己建立标准曲线。
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