产品概况
货号 | EK1547 |
---|---|
产品名称 | Mouse BCAM ELISA Kit |
产品描述 | For quantitative detection of mouse BCAM in , cell lysates |
免疫原 | NSO, E26-A541 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <15pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | BCAM |
基因全名 | basal cell adhesion molecule (Lutheran blood group) |
Uniprot ID | Q9R069 |
指标别名 | AU | Bcam | CD239 | CD239 antigen | F8/G253 antigen | LU | Lutheran | MSK19 | Q9R069 |
背景资料 | Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene. |
显色时间 | 15-20分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1547-CAP | 96-well plate precoated with anti- mouse BCAM antibody | 1 |
EK1547-ST | Lyophilized recombinant mouse BCAM standard | 10ng/tube×2 |
EK1547-DA | Biotinylated anti- mouse BCAM antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 249 | 1119 | 4785 | 243 | 1197 | 4813 |
Standard deviation | 15.93 | 87.28 | 330.16 | 16.52 | 100.54 | 360.97 |
CV(%) | 6.4% | 7.8% | 6.9% | 6.8% | 8.4% | 7.5% |
Mouse BCAM ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 15-20分钟)
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.020 | 0.084 | 0.155 | 0.277 | 0.494 | 0.888 | 1.520 | 2.123 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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