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产品概况
| 货号 | EK0644 |
|---|---|
| 产品名称 | Human CD105 ELISA Kit |
| 产品描述 | For quantitative detection of human CD105 in , cell lysates |
| 免疫原 | CHO, E26-G586 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <15pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | ENG |
| 基因全名 | endoglin |
| Uniprot ID | P17813 |
| 指标别名 | AI528660|AI662476|CD 105|CD105 antigen|Cell surface MJ7/18 antigen|END|Endoglin|ENG|FLJ41744|HHT1|ORW|ORW1|Osler Rendu Weber syndrome 1|OTTMUSP00000012838|OTTMUSP00000012839|RP11 228B15.2|RP23 17P12.4|S endoglin|Transmembrane glycoprotein |
| 背景资料 | CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta(TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0644-CAP | 96-well plate precoated with anti- human CD105 antibody | 1 |
| EK0644-ST | Lyophilized recombinant human CD105 standard | 10ng/tubex2 |
| EK0644-DA | Biotinylated anti- human CD105 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 312 | 1384 | 4821 | 316 | 1275 | 4955 |
| Standard deviation | 16.22 | 74.73 | 318.18 | 17.38 | 72.67 | 386.49 |
| CV(%) | 5.2% | 5.4% | 6.6% | 5.5% | 5.7% | 7.8% |
Human CD105 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.011 | 0.258 | 0.471 | 0.766 | 1.312 | 1.658 | 2.122 | 2.480 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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