产品概况
货号 | EK1216 |
---|---|
产品名称 | Human CD13 ELISA Kit |
产品描述 | For quantitative detection of Human CD13 in , cell lysates |
免疫原 | NSO, K69-K967 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | ANPEP |
基因全名 | alanyl (membrane) aminopeptidase |
Uniprot ID | P15144 |
指标别名 | Alanyl (membrane) aminopeptidase|Alanyl aminopeptidase|Alanyl membrane aminopeptidase|Aminopeptidase M|Aminopeptidase N|AMPN_HUMAN|ANPEP|AP M|AP N|AP-M|AP-N|APN|CD 13|CD13|CD13 antigen|gp150|hAPN|Lap 1|Lap1|Microsomal aminopeptidase|Myeloid plasma membrane glycoprotein CD13|p150|PEPN |
背景资料 | Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility. |
显色时间 | 20-25分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1216-CAP | 96-well plate precoated with anti- human CD13 antibody | 1 |
EK1216-ST | Lyophilized recombinant human CD13 standard | 20ng/tube×2 |
EK1216-DA | Biotinylated anti- human CD13 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 510 | 1565 | 9321 | 554 | 1498 | 9677 |
Standard deviation | 26.01 | 106.42 | 447.4 | 37.67 | 119.84 | 493.52 |
CV(%) | 5.1% | 6.8% | 4.8% | 6.8% | 8% | 5.1% |
Human CD13 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 20-25分钟)
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.027 | 0.122 | 0.183 | 0.336 | 0.584 | 1.098 | 1.807 | 2.273 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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