产品概况
货号 | EK1487 |
---|---|
产品名称 | Human Afamin ELISA Kit |
产品描述 | For quantitative detection of human Afamin in , serum and urine. |
免疫原 | NSO, L22-N599 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | AFM |
基因全名 | afamin |
Uniprot ID | P43652 |
指标别名 | Afamin | Afm | ALB 2 | ALB2 | ALBA | ALF | Alpha albumin | Alpha-albumin | Alpha-Alb | P43652 |
背景资料 | Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1487-CAP | 96-well plate precoated with anti- human Afamin antibody | 1 |
EK1487-ST | Lyophilized recombinant human Afamin standard | 2ng/tube×2 |
EK1487-DA | Biotinylated anti- human Afamin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 54 | 287 | 838 | 49 | 308 | 783 |
Standard deviation | 2.75 | 18.94 | 61.17 | 2.84 | 25.87 | 60.29 |
CV(%) | 5.1% | 6.6% | 7.3% | 5.8% | 8.4% | 7.7% |
Human Afamin ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.030 | 0.149 | 0.251 | 0.480 | 0.751 | 1.261 | 1.806 | 2.289 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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