产品概况
货号 | EK1299 |
---|---|
产品名称 | Rat CD36 ELISA Kit |
产品描述 | For quantitative detection of rat CD36 in , cell lysates |
免疫原 | CHO, G30-K439 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | CD36 |
基因全名 | CD36 molecule (thrombospondin receptor) |
Uniprot ID | Q07969 |
指标别名 | CD36 antigen|CD36 antigen (collagen type I receptor thrombospondin receptor)|CD36 molecule|CD36 molecule (thrombospondin receptor)|CHDS7|Cluster determinant 36|FAT|Fatty acid translocase|Glycoprotein IIIb|GP3B|GP4|GPIIIB|GPIV|Leukocyte differentiation antigen CD36|PAS 4 protein|PAS IV|PASIV|Platelet collagen receptor|Platelet glycoprotein 4|Platelet glycoprotein IV|SCARB3|Scavenger receptor class B member 3|Thrombospondin receptor |
背景资料 | CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1299-CAP | 96-well plate precoated with anti- rat CD36 antibody | 1 |
EK1299-ST | Lyophilized recombinant rat CD36 standard | 10ng/tube×2 |
EK1299-DA | Biotinylated anti- rat CD36 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 297 | 1163 | 5833 | 317 | 1160 | 5856 |
Standard deviation | 16.92 | 91.87 | 425.8 | 19.65 | 92.8 | 462.62 |
CV(%) | 5.7% | 7.9% | 7.3% | 6.2% | 8% | 7.9% |
Rat CD36 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.072 | 0.182 | 0.289 | 0.435 | 0.689 | 1.087 | 1.539 | 2.137 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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