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产品概况
| 货号 | EK1303 |
|---|---|
| 产品名称 | Mouse CD22 ELISA Kit |
| 产品描述 | For quantitative detection of mouse CD22 in , cell lysates |
| 免疫原 | NSO, S22-R702 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | CD22 |
| 基因全名 | CD22 molecule |
| Uniprot ID | P35329 |
| 指标别名 | B cell receptor CD22 precursor|B lymphocyte cell adhesion molecule|B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL CAM|BL-CAM|BLCAM|CD 22|CD22|CD22 antigen|CD22 molecule|CD22 protein|CD22_HUMAN|Lectin 2|Leu14|Lyb8|MGC130020|sialic acid binding Ig like lectin 2|Sialic acid binding immunoglobulin like lectin 2|Sialic acid-binding Ig-like lectin 2|SIGLEC 2|Siglec-2|SIGLEC2|T cell surface antigen Leu 14|T-cell surface antigen Leu-14 |
| 背景资料 | CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors(BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1303-CAP | 96-well plate precoated with anti- mouse CD22 antibody | 1 |
| EK1303-ST | Lyophilized recombinant mouse CD22 standard | 10ng/tube×2 |
| EK1303-DA | Biotinylated anti- mouse CD22 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 240 | 2070 | 4058 | 246 | 1914 | 3971 |
| Standard deviation | 16.56 | 124.2 | 182.61 | 18.94 | 132.06 | 218.4 |
| CV(%) | 6.9% | 6% | 4.5% | 7.7% | 6.9% | 5.5% |
Mouse CD22 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.039 | 0.080 | 0.116 | 0.221 | 0.394 | 0.701 | 1.150 | 1.992 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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