产品概况
货号 | EK0686 |
---|---|
产品名称 | Human CCL18 ELISA Kit |
产品描述 | For quantitative detection of human CCL18 in and serum. |
免疫原 | E.coli, A21-A89 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 7.8pg/ml-500pg/ml |
基因名称 | CCL18 |
基因全名 | chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated) |
Uniprot ID | P55774 |
指标别名 | Alternative macrophage activation associated CC chemokine 1|Alternative macrophage activation-associated CC chemokine 1|AMAC 1|AMAC-1|AMAC1|C C motif chemokine 18|CC chemokine ligand 18|CC chemokine PARC|CCL18|CCL18(4-69)|CCL18_HUMAN|Chemokine (C C motif) ligand 18|Chemokine (C C) dendritic|Chemokine ligand 18|CKb7|DC CK1|DC-CK1|DCCK1|Dendritic cell chemokine 1|Macrophage inflammatory protein 4|MIP 4|MIP-4|MIP4|PARC|Pulmonary and activation regulated chemokine|Pulmonary and activation-regulated chemokine|Small inducible cytokine A18|Small-inducible cytokine A18 |
背景资料 | Chemokine(C-C motif) ligand 18(CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC(pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0686-CAP | 96-well plate precoated with anti- human CCL18 antibody | 1 |
EK0686-ST | Lyophilized recombinant human CCL18 standard | 10ng/tubex2 |
EK0686-DA | Biotinylated anti- human CCL18 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 18 | 49 | 269 | 18 | 52 | 279 |
Standard deviation | 0.99 | 3.52 | 20.17 | 1.29 | 4.62 | 22.59 |
CV(%) | 5.5% | 7.2% | 7.5% | 7.2% | 8.9% | 8.1% |
Human CCL18 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.006 | 0.045 | 0.075 | 0.186 | 0.377 | 0.754 | 1.505 | 2.145 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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