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产品概况
| 货号 | EK0555 |
|---|---|
| 产品名称 | Human 6Ckine ELISA Kit |
| 产品描述 | For quantitative detection of human CCL21 in , cell lysates |
| 免疫原 | E.coli, S24-P134 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <12pg/ml |
| 检测范围 | 62.5pg/ml-4000pg/ml |
| 基因名称 | CCL21 |
| 基因全名 | chemokine (C-C motif) ligand 21 |
| Uniprot ID | O00585 |
| 指标别名 | 6Ckine|Beta chemokine exodus 2|C-C motif chemokine 21|Chemokine (C-C motif) ligand 21|CKb9|ECL|Efficient Chemoattractant for Lymphocytes|Exodus2|MGC34555|OTTHUMP00000000526|OTTHUMP00000000527|OTTHUMP00000021303|SCYA 21|SCYA21|Secondary lymphoid tissue chemokine|SLC|Small inducible cytokine A21|Small inducible cytokine subfamily A (Cys-Cys), member 21|TCA 4|TCA4 |
| 背景资料 | Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0555-CAP | 96-well plate precoated with anti- human CCL21 antibody | 1 |
| EK0555-ST | Lyophilized recombinant human CCL21 standard | 10ng/tubex2 |
| EK0555-DA | Biotinylated anti- human CCL21 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 100 | 709 | 1352 | 103 | 706 | 1475 |
| Standard deviation | 5.6 | 28.36 | 82.47 | 6.9 | 31.77 | 92.92 |
| CV(%) | 5.6% | 4% | 6.1% | 6.7% | 4.5% | 6.3% |
Human 6Ckine ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.031 | 0.057 | 0.102 | 0.203 | 0.408 | 0.504 | 1.178 | 2.318 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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