产品概况
货号 | EK0690 |
---|---|
产品名称 | Human CCL28 ELISA Kit |
产品描述 | For quantitative detection of human CCL28 in , cell lysates |
免疫原 | E.coli, I23-Y127 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | CCL28 |
基因全名 | chemokine (C-C motif) ligand 28 |
Uniprot ID | Q9NRJ3 |
指标别名 | CCK 1|CCK1|CCL 28|Chemokine (C-C motif) ligand 28|MEC|MGC71902|Mucosae associated epithelial chemokine|OTTHUMP0000022162|SCYA28|Small inducible cytokine A28|Small inducible cytokine A28 [Precursor]|Small inducible cytokine subfamily A (Cys-Cys), member 28 |
背景资料 | CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans. |
显色时间 | 15-20分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0690-CAP | 96-well plate precoated with anti- human CCL28 antibody | 1 |
EK0690-ST | Lyophilized recombinant human CCL28 standard | 10ng/tube×2 |
EK0690-DA | Biotinylated anti- human CCL28 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 73 | 277 | 866 | 77 | 254 | 867 |
Standard deviation | 5.03 | 21.32 | 59.75 | 5.77 | 20.32 | 64.15 |
CV(%) | 6.9% | 7.7% | 6.9% | 7.5% | 8% | 7.4% |
Human CCL28 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 15-20分钟)
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.019 | 0.137 | 0.275 | 0.476 | 0.857 | 1.532 | 2.039 | 2.324 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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