产品概况
货号 | EK0708 |
---|---|
产品名称 | Mouse CD80 ELISA Kit |
产品描述 | For quantitative detection of mouse CD80 in and serum. |
免疫原 | NSO, D37-K245 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | CD80 |
基因全名 | CD80 molecule |
Uniprot ID | Q00609 |
指标别名 | Activation B7-1 antigen|B7|BB1|CD80|CD80_HUMAN|CTLA-4 counter-receptor B7.1|T-lymphocyte activation antigen CD80 |
背景资料 | The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86(601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1, D37—K245, which is composed of two single chains acids with the dipolymer. |
显色时间 | 15-20分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0708-CAP | 96-well plate precoated with anti- mouse CD80 antibody | 1 |
EK0708-ST | Lyophilized recombinant mouse CD80 standard | 10ng/tube×2 |
EK0708-DA | Biotinylated anti- mouse CD80 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 153 | 733 | 1704 | 158 | 717 | 1630 |
Standard deviation | 9.94 | 47.64 | 132.91 | 13.27 | 59.51 | 133.66 |
CV(%) | 6.5% | 6.5% | 7.8% | 8.4% | 8.3% | 8.2% |
Mouse CD80 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 15-20分钟)
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.001 | 0.082 | 0.174 | 0.333 | 0.609 | 0.997 | 1.508 | 2.112 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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