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产品概况
| 货号 | EK0909 |
|---|---|
| 产品名称 | Human Aggrecan ELISA Kit |
| 产品描述 | For quantitative detection of human Aggrecan in , serum and urine. |
| 免疫原 | NSO, V20-G675 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <50pg/ml |
| 检测范围 | 1.56ng/ml-100ng/ml |
| 基因名称 | ACAN |
| 基因全名 | aggrecan |
| Uniprot ID | P16112 |
| 指标别名 | ACAN|AGC 1|AGC1|AGCAN|Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122)|Aggrecan 1|Aggrecan core protein|Aggrecan proteoglycan|Aggrecan structural proteoglycan of cartilage|Aggrecan1| ATEGQV|Cartilage specific proteoglycan core protein|Chondroitin sulfate proteoglycan 1|Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122|Chondroitin sulfate proteoglycan core protein 1|CSPG 1| CSPG1|CSPGCP|JSCATE|Large aggregating proteoglycan|mcspg|mgsk16|MSK 16|MSK16|SEDK |
| 背景资料 | Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0909-CAP | 96-well plate precoated with anti- human Aggrecan antibody | 1 |
| EK0909-ST0 | Lyophilized recombinant human Aggrecan standard | 100ng/tube×2 |
| EK0909-DA | Biotinylated anti- human Aggrecan antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
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1.96孔读板仪器。
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2.自动洗板机.
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3.移液器和枪头。建议使用多通道移液器/排枪。
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4.Eppendorf 管。
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5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
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0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
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0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
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*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 3084 | 13064 | 43102 | 3322 | 13982 | 40208 |
| Standard deviation | 240.55 | 679.32 | 1939.59 | 275.72 | 908.83 | 1809.36 |
| CV(%) | 7.8% | 5.2% | 4.5% | 8.3% | 6.5% | 4.5% |
Human Aggrecan ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)
| Concentration(ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.004 | 0.069 | 0.175 | 0.333 | 0.559 | 1.021 | 1.672 | 2.054 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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