产品概况
货号 | EK0570 |
---|---|
产品名称 | Mouse CD30 ELISA Kit |
产品描述 | For quantitative detection of mouse CD30 in and serum. |
免疫原 | NSO, F19-T281 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | TNFRSF8 |
基因全名 | tumor necrosis factor receptor superfamily, member 8 |
Uniprot ID | Q60846 |
指标别名 | CD 30|CD153|CD30|CD30 antigen|CD30L receptor|Cytokine receptor CD30|D1S166E|KI 1|Ki 1 antigen|Ki-1 antigen|KI1|Lymphocyte activation antigen CD30|TNFRSF 8|TNFRSF8|TNFRSF8|TNR8_HUMAN|Tumor Necrosis Factor Receptor Superfamily Member 8|Tumor Necrosis Factor Receptor Superfamily Member 8 |
背景资料 | CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0570-CAP | 96-well plate precoated with anti- mouse CD30 antibody | 1 |
EK0570-ST | Lyophilized recombinant mouse CD30 standard | 10ng/tubex2 |
EK0570-DA | Biotinylated anti- mouse CD30 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 40 | 255 | 414 | 42 | 257 | 418 |
Standard deviation | 2.6 | 16.83 | 17.8 | 3.19 | 20.56 | 25.49 |
CV(%) | 6.5% | 6.6% | 4.3% | 7.6% | 8% | 6.1% |
Mouse CD30 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.014 | 0.081 | 0.148 | 0.235 | 0.505 | 1.010 | 1.482 | 2.158 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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