Mouse Cadherin-2/N-Cadherin ELISA Kit

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货号：EK1155

反应性：Mouse

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK1155-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK1155
产品名称	Mouse CDH2/N-Cadherin ELISA Kit
产品描述	For quantitative detection of mouse Cadherin-2 in , cell lysates
免疫原	NSO, D160-A724
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<20pg/ml
检测范围	0.78ng/ml-50ng/ml
基因名称	CDH2
基因全名	cadherin 2, type 1, N-cadherin (neuronal)
Uniprot ID	P15116
指标别名	CDw325\|CDHN\|Neural cadherin\|N-cadherin\|CD325
背景资料	Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
显色时间	20-25分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1155-CAP	96-well plate precoated with anti- mouse Cadherin-2 antibody	1
EK1155-ST	Lyophilized recombinant mouse Cadherin-2 standard	50ng/tube×2
EK1155-DA	Biotinylated anti- mouse Cadherin-2 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Mouse CDH2/N-Cadherin ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 20-25分钟)

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.081	0.181	0.249	0.456	0.675	1.284	1.864	2.330

*典型数据仅供参考。用户需要自己建立标准曲线。

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