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产品概况
| 货号 | EK1127 |
|---|---|
| 产品名称 | Human CCL16 ELISA Kit |
| 产品描述 | For quantitative detection of human CCL16 in , cell lysates |
| 免疫原 | E.coli, Q24-Q120 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 7.8pg/ml-500pg/ml |
| 基因名称 | CCL16 |
| 基因全名 | chemokine (C-C motif) ligand 16 |
| Uniprot ID | O15467 |
| 指标别名 | C-C motif chemokine 16|CCL16|CCL16_HUMAN|Chemokine CC-4|Chemokine LEC|CKb12|HCC-4|HCC4|IL-10-inducible chemokine|IL10 inducible chemokine|ILINCK|LCC-1|LCC1|Liver CC chemokine 1 precursor|Liver expressed chemokine|Liver-expressed chemokine|LMC|Lymphocyte and monocyte chemoattractant|Monotactin 1|Monotactin-1|MTN-1|Mtn1|NCC-4|NCC4|New CC chemokine 4|SCYA16|SCYL4|Small inducible cytokine A16 precursor|Small inducible cytokine subfamily A (Cys Cys) member 16|Small-inducible cytokine A16 |
| 背景资料 | Chemokine(C-C motif) ligand 16(CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine(LEC) and Monotactin-1(MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8. |
| 显色时间 | 15-20 分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1127-CAP | 96-well plate precoated with anti- human CCL16 antibody | 1 |
| EK1127-ST | Lyophilized recombinant human CCL16 standard | 10ng/tube×2 |
| EK1127-DA | Biotinylated anti- human CCL16 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 17 | 60 | 271 | 18 | 54 | 267 |
| Standard deviation | 0.85 | 3.18 | 20.86 | 1.02 | 3.56 | 21.36 |
| CV(%) | 5% | 5.3% | 7.7% | 5.7% | 6.6% | 8% |
Human CCL16 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20 分钟)
| Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.024 | 0.070 | 0.117 | 0.200 | 0.384 | 0.777 | 1.422 | 2.495 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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