Rat CXCL2/MIP-2 ELISA Kit

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货号：EK0725

反应性：Rat

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
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产品概况

货号	EK0725
产品名称	Rat CXCL2/MIP-2 ELISA Kit
产品描述	For quantitative detection of rat MIP-2 in , cell lysates
免疫原	E.coli, S32-N100
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	15.6pg/ml-1000pg/ml
基因名称	CXCL2
基因全名	chemokine (C-X-C motif) ligand 2
Uniprot ID	P30348
指标别名	C-X-C motif chemokine 2\|Chemokine (C-X-C motif) ligand 2\|CINC-2a\|Cxcl2\|CXCL2_HUMAN\|Gro beta\|Gro-beta\|GRO-beta(5-73)\|GRO-beta-T\|GRO2\| GRO2 oncogene\|GROb\|Growth-regulated protein beta\|Hematopoietic synergistic factor\|HSF\|Macrophage inflammatory protein 2-alpha\|Melanoma growth stimulatory activity beta\|MGSA beta\|MGSA-b\|MIP 2\|MIP 2a\|MIP2\|MIP2-alpha\|MIP2A\|SB-251353\|SCYB2
背景资料	MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.
显色时间	15-20分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0725-CAP	96-well plate precoated with anti- rat MIP-2 antibody	1
EK0725-ST	Lyophilized recombinant rat MIP-2 standard	10ng/tube×2
EK0725-DA	Biotinylated anti- rat MIP-2 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Rat CXCL2/MIP-2 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 15-20分钟)

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.059	0.124	0.156	0.244	0.451	0.684	1.346	1.916

*典型数据仅供参考。用户需要自己建立标准曲线。

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