产品概况
货号 | EK0725 |
---|---|
产品名称 | Rat CXCL2/MIP-2 ELISA Kit |
产品描述 | For quantitative detection of rat MIP-2 in , cell lysates |
免疫原 | E.coli, S32-N100 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | CXCL2 |
基因全名 | chemokine (C-X-C motif) ligand 2 |
Uniprot ID | P30348 |
指标别名 | C-X-C motif chemokine 2|Chemokine (C-X-C motif) ligand 2|CINC-2a|Cxcl2|CXCL2_HUMAN|Gro beta|Gro-beta|GRO-beta(5-73)|GRO-beta-T|GRO2| GRO2 oncogene|GROb|Growth-regulated protein beta|Hematopoietic synergistic factor|HSF|Macrophage inflammatory protein 2-alpha|Melanoma growth stimulatory activity beta|MGSA beta|MGSA-b|MIP 2|MIP 2a|MIP2|MIP2-alpha|MIP2A|SB-251353|SCYB2 |
背景资料 | MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells. |
显色时间 | 15-20分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0725-CAP | 96-well plate precoated with anti- rat MIP-2 antibody | 1 |
EK0725-ST | Lyophilized recombinant rat MIP-2 standard | 10ng/tube×2 |
EK0725-DA | Biotinylated anti- rat MIP-2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 35 | 171 | 537 | 31 | 178 | 563 |
Standard deviation | 2.69 | 7.86 | 21.48 | 2.88 | 8.36 | 27.02 |
CV(%) | 7.7% | 4.6% | 4% | 9.3% | 4.7% | 4.8% |
Rat CXCL2/MIP-2 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 15-20分钟)
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.059 | 0.124 | 0.156 | 0.244 | 0.451 | 0.684 | 1.346 | 1.916 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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