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产品概况
| 货号 | EK0924 |
|---|---|
| 产品名称 | Mouse FCER2/CD23 ELISA Kit |
| 产品描述 | For quantitative detection of mouse CD23 in , cell lysates |
| 免疫原 | NSO, E50-P331 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <15pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | FCER2 |
| 基因全名 | Fc fragment of IgE, low affinity II, receptor for (CD23) |
| Uniprot ID | P20693 |
| 指标别名 | BLAST 2|BLAST-2|C-type lectin domain family 4 member J|CD23|CD23 antigen|CD23A|CLEC4J|Fc fragment of IgE low affinity II receptor for (CD23)|Fc-epsilon-RII|FCE2|Fcer2|FCER2_HUMAN|IGEBF|Immunoglobulin E-binding factor|Low affinity immunoglobulin epsilon Fc receptor|Low affinity immunoglobulin epsilon Fc receptor soluble form|Lymphocyte IgE receptor |
| 背景资料 | CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0924-CAP | 96-well plate precoated with anti- mouse CD23 antibody | 1 |
| EK0924-ST | Lyophilized recombinant mouse CD23 standard | 10ng/tube×2 |
| EK0924-DA | Biotinylated anti- mouse CD23 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 256 | 1831 | 5853 | 234 | 1881 | 5493 |
| Standard deviation | 11 | 137.32 | 345.32 | 12.87 | 156.12 | 373.52 |
| CV(%) | 4.3% | 7.5% | 5.9% | 5.5% | 8.3% | 6.8% |
Mouse FCER2/CD23 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.021 | 0.045 | 0.137 | 0.279 | 0.537 | 1.009 | 1.503 | 2.141 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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