产品概况
货号 | EK1608 |
---|---|
产品名称 | Mouse BMP6 ELISA Kit |
产品描述 | For Quantitative Detection of mouse BMP-6 in bone tissue, and serum. |
免疫原 | CHO, S372-H510 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | Bmp6 |
基因全名 | bone morphogenetic protein 6 |
Uniprot ID | P20722 |
指标别名 | Bone morphogenetic protein 6|BMP-6|VG-1-related protein|VGR-1|Bmp6|Bmp-6|Vgr1 |
背景资料 | Bone morphogenetic protein 6 is a protein that in humans is encoded by the BMP6 gene. It is mapped to 6p24-p23. The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1608-CAP | 96-well plate precoated with anti- mouse BMP-6 antibody | 1 |
EK1608-ST | Lyophilized recombinant mouse BMP-6 standard | 10ng/tube×2 |
EK1608-DA | Biotinylated anti- mouse BMP-6 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 214 | 1829 | 5790 | 226 | 1673 | 5512 |
Standard deviation | 12.41 | 74.98 | 399.51 | 13.33 | 86.99 | 391.35 |
CV(%) | 5.8% | 4.1% | 6.9% | 5.9% | 5.2% | 7.1% |
Mouse BMP6 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.113 | 0.172 | 0.226 | 0.347 | 0.547 | 0.957 | 1.533 | 2.373 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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