产品概况
货号 | EK1587 |
---|---|
产品名称 | Mouse CA9 ELISA Kit |
产品描述 | For quantitative detection of mouse CA9 in , serum, plasma (heparin, EDTA, citrate) and urine. |
免疫原 | NSO, Q32-D390 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | Ca9 |
基因全名 | carbonic anhydrase 9 |
Uniprot ID | Q8VHB5 |
指标别名 | Ca9 | CAIX | MN/CA9 | CA-IX | Car9 | Q16790 |
背景资料 | Carbonic anhydrase 9 (CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1587-CAP | 96-well plate precoated with anti- mouse CA9 antibody | 1 |
EK1587-ST | Lyophilized recombinant mouse CA9 standard | 10ng/tube×2 |
EK1587-DA | Biotinylated anti- mouse CA9 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 34 | 186 | 448 | 35 | 180 | 461 |
Standard deviation | 2.55 | 12.27 | 28.67 | 2.73 | 13.32 | 35.49 |
CV(%) | 7.5% | 6.6% | 6.4% | 7.8% | 7.4% | 7.7% |
Mouse CA9 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.042 | 0.145 | 0.214 | 0.345 | 0.581 | 1.14 | 1.612 | 2.125 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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