Mouse CA9 ELISA Kit

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货号：EK1587

反应性：Mouse

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK1587-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK1587
产品名称	Mouse CA9 ELISA Kit
产品描述	For quantitative detection of mouse CA9 in , serum, plasma (heparin, EDTA, citrate) and urine.
免疫原	NSO, Q32-D390
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	15.6pg/ml-1000pg/ml
基因名称	Ca9
基因全名	carbonic anhydrase 9
Uniprot ID	Q8VHB5
指标别名	Ca9 \| CAIX \| MN/CA9 \| CA-IX \| Car9 \| Q16790
背景资料	Carbonic anhydrase 9 (CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
显色时间	25-30分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1587-CAP	96-well plate precoated with anti- mouse CA9 antibody	1
EK1587-ST	Lyophilized recombinant mouse CA9 standard	10ng/tube×2
EK1587-DA	Biotinylated anti- mouse CA9 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Mouse CA9 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 25-30分钟)

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.042	0.145	0.214	0.345	0.581	1.14	1.612	2.125

*典型数据仅供参考。用户需要自己建立标准曲线。

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