Human CD200 ELISA Kit

货号:EK0993
反应性:Human
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产品概况

货号 EK0993
产品名称 Human CD200 ELISA Kit
产品描述 For quantitative detection of human CD200 in , cell lysates
免疫原 NSO, Q31-G232
交叉反应 There is no detectable cross-reactivity with other relevant proteins.
敏感性 <10pg/ml
检测范围 31.2pg/ml-2000pg/ml
基因名称 CD200
基因全名 CD200 molecule
Uniprot ID P41217
指标别名 antigen identified by monoclonal antibody MRC OX-2|CD_antigen=CD200|CD200 antigen|MOX1|MOX2|MRC OX-2 antigen|My033|OX-2 membrane glycoprotein
背景资料 OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
显色时间 20-25分钟

试剂盒内容

SKU 名称 数量
Catalog number Description Quantity
EK0993-CAP 96-well plate precoated with anti- human CD200 antibody 1
EK0993-ST Lyophilized recombinant human CD200 standard 10ng/tube×2
EK0993-DA Biotinylated anti- human CD200 antibody 100μl(dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 100μl(dilution 1:100)
AR1106-1 Sample diluent buffer 30 ml
AR1106-2 Antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

  • 1.96孔读板仪器。
  • 2.自动洗板机.
  • 3.移液器和枪头。建议使用多通道移液器/排枪。
  • 4.Eppendorf 管。
  • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
    • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 80 246 894 73 239 980
Standard deviation 3.76 12.54 68.83 4.16 14.81 77.42
CV(%) 4.7% 5.1% 7.7% 5.7% 6.2% 7.9%

Human CD200 ELISA Kit的典型数据示例(取自最近的一批次质检数据)

(TMB 孵育条件: 37°C, 20-25分钟)

Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000
O.D. 0.034 0.099 0.135 0.198 0.354 0.676 1.106 2.093

*典型数据仅供参考。用户需要自己建立标准曲线。

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