Mouse beta IG-H3/TGFBI ELISA Kit

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货号：EK1191

反应性：Mouse

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK1191-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK1191
产品名称	Mouse beta IG-H3/TGFBI ELISA Kit
产品描述	For quantitative detection of mouse beta IG-H3 in , cell lysates
免疫原	NSO, G24-H683
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<5pg/ml
检测范围	156pg/ml-10000pg/ml
基因名称	TGFBI
基因全名	transforming growth factor, beta-induced, 68kDa
Uniprot ID	P82198
指标别名	Beta ig \| Beta ig h3 \| Beta ig-h3 \| Big h3 \| BIGH3 \| CDB1 \| CDG2 \| CDGG1 \| CSD \| CSD1 \| CSD2 \| CSD3 \| EBMD \| Kerato epithelin \| Kerato-epithelin \| LCD1 \| RGD CAP \| RGD-CAP \| TGFBI \| Q15582
背景资料	Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
显色时间	15-20 分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1191-CAP	96-well plate precoated with anti- mouse beta IG-H3 antibody	1
EK1191-ST	Lyophilized recombinant mouse beta IG-H3 standard	10ng/tube×2
EK1191-DA	Biotinylated anti- mouse beta IG-H3 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Mouse beta IG-H3/TGFBI ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 15-20 分钟)

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.004	0.036	0.071	0.147	0.321	0.733	1.373	2.509

*典型数据仅供参考。用户需要自己建立标准曲线。

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