Human CCL13/MCP4 ELISA Kit

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货号：EK0809

反应性：Human

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK0809-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK0809
产品名称	Human MCP-4 ELISA Kit
产品描述	For quantitative detection of human CCL13 in , cell lysates
免疫原	E.coli, Q24-T98
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	7.8pg/ml-500pg/ml
基因名称	CCL13
基因全名	chemokine (C-C motif) ligand 13
Uniprot ID	Q99616
指标别名	C-C motif chemokine 13\|CCL13\|CCL13_HUMAN\|Chemokine (C C motif) ligand 13\|CK beta 10\|CK-beta-10\|CKb10\|Macrophage/monocyte chemotactic protein 4\|MCP-4\|MGC17134\|Monocyte chemoattractant protein 4\|Monocyte chemotactic protein 4\|NCC 1\|NCC-1\|NCC1\|New CC chemokine 1\|SCYA13\|SCYL1\|short chain\|Small inducible cytokine A13\|Small inducible cytokine A13 precursor\|Small inducible cytokine subfamily A (Cys Cys) member 13\|Small-inducible cytokine A13
背景资料	Chemokine(C-C motif) ligand 13(CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.
显色时间	20-25分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0809-CAP	96-well plate precoated with anti- human CCL13 antibody	1
EK0809-ST	Lyophilized recombinant human CCL13 standard	10ng/tube×2
EK0809-DA	Biotinylated anti- human CCL13 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Human MCP-4 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 20-25分钟)

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.004	0.025	0.054	0.116	0.254	0.636	1.469	2.245

*典型数据仅供参考。用户需要自己建立标准曲线。

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