•     · 快速方便的单管反应
•     · 适合各种RNA模板，无需优化反应条件
•     · 独特的酶组合，确保反应的高特异性和高灵敏度
•     · 经优化的缓冲体系确保了高效的逆转录和扩增过程

QIAGEN One-Step RT-PCR Kit包含Omniscript逆转录酶和Sensiscript逆转录酶的混合物、HotStarTaq DNA Polymerase、QIAGEN OneStep RT-PCR Buffer、dNTP混合液和Q-Solution。Q-Solution是一种新型的辅助剂，有助于实现“困难”模板（如：高GC含量的模板）的高效扩增。简单的单管反应体系构建和优化的试剂盒组分确保了结果成功且灵敏度高。

QIAGEN OneStep RT-PCR Kit提供方便的规格，可使用RNA模板进行高灵敏度和特异性的RT-PCR。试剂盒中包含优化的组分，可在同一反应混合液中进行逆转录和PCR扩增，完成"一步式"反应。独特的酶组合和专门研发的反应缓冲液确保在一个管中进行高效、高特异性的逆转录和PCR，无需优化（参见" Highly specific RT-PCR using low amounts of template" 和 " Efficient detection of viral RNA"）。试剂盒提供的新型的、双阳离子PCR缓冲液确保在宽退火温度范围内得到高产量的特异性PCR产物（参见" Influence of annealing temperature on specificity"）。使用Q-Solution可提高不理想的RT-PCR的表现，这种独特的辅助剂可帮助逆转录并高效扩增具有高GC含量和复杂二级结构的模板（参见" RT-PCR of GC-rich template"）。

Highly specific RT-PCR using low amounts of template.

One-step RT-PCR was carried out using the indicated amounts of total RNA from HeLa cells and primers specific for α-catenin, amplifying a 690 bp product. All reactions were carried out following suppliers' instructions. M: 100 bp ladder.

fficient detection of viral RNA.

A 336 bp fragment of F-gene mRNA was reverse-transcribed and amplified from Sendai virus RNA isolated from persistently infected Vero cells. Reactions were prepared using the QIAGEN OneStep RT-PCR Kit and the indicated number of viral genome copies. M: markers. (Data kindly provided by H. Rausch, Max Planck Institute for Biochemistry, Martinsried, Germany as part of the project "Experimental control of virological work at safety levels 2 and 3 in Bavaria," supported by the Bavarian Ministry of the Environment.)

Influence of annealing temperature on specificity.

One-step RT-PCR was performed using kits from the indicated suppliers over a range of annealing temperatures. A 1289 bp fragment from the human RCC1 gene was reverse transcribed and amplified from Hela RNA (arrow). M: markers. High levels of specific amplification without optimization were observed only with the QIAGEN OneStep RT-PCR Kit.

RT-PCR of GC-rich template.

A 439 bp fragment of transcription factor TAFII100 mRNA (GC content: 75%) was reverse-transcribed and amplified from HeLa-cell total RNA. Reactions were prepared using the QIAGEN OneStep RT-PCR Kit with (+) or without (–) Q-Solution. Q-Solution ensures specific amplification of GC-rich templates. M: markers.

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