Human CCL15 ELISA Kit

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货号：EK1120

反应性：Human

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK1120-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK1120
产品名称	Human CCL15 ELISA Kit
产品描述	For quantitative detection of human CCL15 in , cell lysates
免疫原	E.coli, Q22-I113
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	15.6pg/ml-1000pg/ml
基因名称	CCL15
基因全名	chemokine (C-C motif) ligand 15
Uniprot ID	Q16663
指标别名	CC chemokine 3\|CCL15\|CCL15(29-92)\|CCL15_HUMAN\|Chemokine CC-2\|Chemokine CC2\|HCC-2\|HCC2\|HMRP 2B\|HMRP2B\|Leukotactin 1\|Leukotactin-1\|LKN-1\|LKN1\|Macrophage inflammatory protein 5\|MIP-1 delta\|MIP-5\|MIP1 delta\|MIP1d\|MIP5\|Mrp-2b\|Mrp2b\|NCC-3\|NCC3\|SCYA15\|SCYL3\|Small inducible cytokine A15\|Small inducible cytokine subfamily A (Cys-Cys), member 15\|Small-inducible cytokine A15\|SY15
背景资料	Chemokine(C-C motif) ligand 15(CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14.
显色时间	15-20分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1120-CAP	96-well plate precoated with anti- human CCL15 antibody	1
EK1120-ST	Lyophilized recombinant human CCL15 standard	10ng/tube×2
EK1120-DA	Biotinylated anti- human CCL15 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Human CCL15 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 15-20分钟)

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.042	0.092	0.121	0.194	0.319	0.570	1.023	2.010

*典型数据仅供参考。用户需要自己建立标准曲线。

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