产品概况
货号 | EK1191 |
---|---|
产品名称 | Mouse beta IG-H3/TGFBI ELISA Kit |
产品描述 | For quantitative detection of mouse beta IG-H3 in , cell lysates |
免疫原 | NSO, G24-H683 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <5pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | TGFBI |
基因全名 | transforming growth factor, beta-induced, 68kDa |
Uniprot ID | P82198 |
指标别名 | Beta ig | Beta ig h3 | Beta ig-h3 | Big h3 | BIGH3 | CDB1 | CDG2 | CDGG1 | CSD | CSD1 | CSD2 | CSD3 | EBMD | Kerato epithelin | Kerato-epithelin | LCD1 | RGD CAP | RGD-CAP | TGFBI | Q15582 |
背景资料 | Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. |
显色时间 | 15-20 分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1191-CAP | 96-well plate precoated with anti- mouse beta IG-H3 antibody | 1 |
EK1191-ST | Lyophilized recombinant mouse beta IG-H3 standard | 10ng/tube×2 |
EK1191-DA | Biotinylated anti- mouse beta IG-H3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 301 | 1595 | 5482 | 302 | 1576 | 5124 |
Standard deviation | 21.37 | 95.7 | 241.2 | 22.65 | 100.86 | 312.56 |
CV(%) | 7.1% | 6% | 4.4% | 7.5% | 6.4% | 6.1% |
Mouse beta IG-H3/TGFBI ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 15-20 分钟)
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.004 | 0.036 | 0.071 | 0.147 | 0.321 | 0.733 | 1.373 | 2.509 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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