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产品概况
| 货号 | EK1118 |
|---|---|
| 产品名称 | Human A2M ELISA Kit |
| 产品描述 | For quantitative detection of human A2M in , cell lysates |
| 免疫原 | from human plasma |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <20pg/ml |
| 检测范围 | 625pg/ml-40000pg/ml |
| 基因名称 | A2M |
| 基因全名 | alpha-2-macroglobulin |
| Uniprot ID | P01023 |
| 指标别名 | C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5|A2m|A2MD|A2MG_HUMAN|Alpha-2-M|Alpha-2-macroglobulin|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5|CPAMD5|FWP007|Pregnancy zone protein|S863-7 |
| 背景资料 | Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease(AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates(activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1118-CAP | 96-well plate precoated with anti- human A2M antibody | 1 |
| EK1118-ST | Lyophilized recombinant human A2M standard | 40ng/tube×2 |
| EK1118-DA | Biotinylated anti- human A2M antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
● 1.96孔读板仪器。
● 2.自动洗板机.
● 3.移液器和枪头。建议使用多通道移液器/排枪。
● 4.Eppendorf 管。
● 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
◆ 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
◆ 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1367 | 3876 | 26784 | 1316 | 3942 | 26889 |
| Standard deviation | 97.05 | 236.43 | 2062.36 | 103.96 | 240.46 | 2151.12 |
| CV(%) | 7.1% | 6.1% | 7.7% | 7.9% | 6.1% | 8% |
Human A2M ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)
| Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
| O.D. | 0.075 | 0.129 | 0.203 | 0.332 | 0.575 | 1.108 | 1.477 | 2.135 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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