产品中心
-
分子生物学
-
细胞生物学
-
蛋白质组学
-
蛋白凝胶纯化
-
生化试剂
-
仪器设备
-
通用实验耗材
-
色谱耗材
浏览历史
产品概况
| 货号 | EK1352 |
|---|---|
| 产品名称 | Mouse ANGPTL3 ELISA Kit |
| 产品描述 | For quantitative detection of mouse ANGPTL3 in , cell lysates |
| 免疫原 | NSO, S17-T455 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | ANGPTL3 |
| 基因全名 | angiopoietin-like 3 |
| Uniprot ID | Q9R182 |
| 指标别名 | ANG 5|ANG-5|ANG5|Angiopoietin 5|Angiopoietin like 3|Angiopoietin related protein 3|Angiopoietin-5|Angiopoietin-like protein 3|Angiopoietin-related protein 3|ANGL3_HUMAN|ANGPT5|ANGPTL3|ANL3|FHBL2|OTTHUMP00000010719|UNQ153/PRO179 |
| 背景资料 | ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1352-CAP | 96-well plate precoated with anti- mouse ANGPTL3 antibody | 1 |
| EK1352-ST | Lyophilized recombinant mouse ANGPTL3 standard | 10ng/tube×2 |
| EK1352-DA | Biotinylated anti- mouse ANGPTL3 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 234 | 1410 | 5833 | 215 | 1499 | 6259 |
| Standard deviation | 15.91 | 59.22 | 274.15 | 17.2 | 79.44 | 337.98 |
| CV(%) | 6.8% | 4.2% | 4.7% | 8% | 5.3% | 5.4% |
Mouse ANGPTL3 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.006 | 0.064 | 0.125 | 0.259 | 0.511 | 0.936 | 1.623 | 2.194 |
*典型数据仅供参考。用户需要自己建立标准曲线。
暂无产品信息
暂无参考文献
暂无文件下载
COA检索
请输入批号:
.png)
