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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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产品概况
| 货号 | A02103-1 |
|---|---|
| 产品名称 | Anti-AGT Antibody |
| 基因名 | AGT |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 52KD |
| 免疫原 | E.coli-derived mouse Angiotensinogen recombinant protein (Position: D25-A115). Mouse Angiotensinogen shares 62.2% and 87.9% amino acid (aa) sequence identity with human and rat Angiotensinogen, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Lyophilized |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | AGT(ANGIOTENSINOGEN), also called SERPINA8, is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. |
| 研究类别 | 1. Abonia, J. P., Abel, K. J., Eddy, R. L., Elliott, R. W., Chapman, V. M., Shows, T. B., Gross, K. W. Linkage of Agt and Actsk-1 to distal mouse chromosome 8 loci: a new conserved linkage. Mammalian Genome 4: 25-32, 1993.2. Arakawa, K., Minohara, A., Yamada, J., Nakamura, M. Enzymatic degradation and electrophoresis of human angiotensin I. Biochim. Biophys. Acta 168: 106-112, 1968.3. Basso N, Terragno NA (December 2001). "History about the discovery of the renin-angiotensin system". Hypertension 38 (6): 1246–9. |
| Uniprot ID | AGT: P11859 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot: | 1:500-2000 |
| Immunohistochemistry in paraffin section: | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最优稀释度需要用户自己调试，此处数据仅供参考。
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