Mouse Marapsin/Pancresin ELISA Kit

货号:EK1481
反应性:Mouse
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产品概况

货号 EK1481
产品名称 Mouse Marapsin/Pancresin ELISA Kit
产品描述 For quantitative detection of mouse Marapsin in and serum.
免疫原 NSO, M38-V328
交叉反应 There is no detectable cross-reactivity with other relevant proteins.
敏感性 <10pg/ml
检测范围 125pg/ml-8000pg/ml
基因名称 PRSS27
基因全名 protease, serine 27
Uniprot ID Q8BJR6
指标别名 CAPH2 | Marapsin | MPN | Pancreasin | Prss27 | Prss 27 | Protease serine 27 | UNQ1884/PRO4327 | Q8BJR6
背景资料 Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.
显色时间 15-20分钟

试剂盒内容

SKU 名称 数量
Catalog number Description Quantity
EK1481-CAP 96-well plate precoated with anti- mouse Marapsin antibody 1
EK1481-ST Lyophilized recombinant mouse Marapsin standard 10ng/tube×2
EK1481-DA Biotinylated anti- mouse Marapsin antibody 100μl(dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 100μl(dilution 1:100)
AR1106-1 Sample diluent buffer 30ml
AR1106-2 Antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

  • 1.96孔读板仪器。
  • 2.自动洗板机.
  • 3.移液器和枪头。建议使用多通道移液器/排枪。
  • 4.Eppendorf 管。
  • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
    • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 178 1127 4357 171 1106 3990
Standard deviation 9.96 85.65 243.99 9.57 98.43 299.25
CV(%) 5.6% 7.6% 5.6% 5.6% 8.9% 7.5%

Mouse Marapsin/Pancresin ELISA Kit的典型数据示例(取自最近的一批次质检数据)

(TMB 孵育条件: 37°C, 15-20分钟)

Concentration(pg/ml) 0 125 250 500 1000 2000 4000 8000
O.D. 0.005 0.074 0.183 0.309 0.618 1.201 1.794 2.133

*典型数据仅供参考。用户需要自己建立标准曲线。

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