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产品概况
| 货号 | EK0827 |
|---|---|
| 产品名称 | Human RAGE ELISA Kit |
| 产品描述 | For quantitative detection of human Rage in , cell lysates |
| 免疫原 | NSO, Q24-A344 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 78pg/ml-5000pg/ml |
| 基因名称 | AGER |
| 基因全名 | advanced glycosylation end product-specific receptor |
| Uniprot ID | Q15109 |
| 指标别名 | Advanced glycosylation end product specific receptor|Advanced glycosylation end product-specific receptor|Ager|DAMA 358M23.4|LE 9211 A antigen |MAPK MAK MRK overlapping kinase |MGC22357|MOK|OTTHUMP00000174885|RAGE 1|RAGE isoform NtRAGE-delta|RAGE isoform sRAGE-delta|RAGE_HUMAN|RAGE1|Receptor for advanced glycation end products|receptor for advanced glycation end-products variant 20|Receptor for advanced glycosylation end products|Renal tumor antigen |Renal tumor antigen 1 |
| 背景资料 | RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as “AGER”. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B(NF-kappaB). |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0827-CAP | 96-well plate precoated with anti- human Rage antibody | 1 |
| EK0827-ST | Lyophilized recombinant human Rage standard | 10ng/tube×2 |
| EK0827-DA | Biotinylated anti- human Rage antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 154 | 623 | 3076 | 159 | 595 | 3344 |
| Standard deviation | 10.16 | 27.41 | 221.47 | 10.65 | 32.72 | 250.8 |
| CV(%) | 6.6% | 4.4% | 7.2% | 6.7% | 5.5% | 7.5% |
Human RAGE ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)
| Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.075 | 0.166 | 0.291 | 0.391 | 0.875 | 1.375 | 1.867 | 2.153 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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