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产品概况
| 货号 | EK0834 |
|---|---|
| 产品名称 | Mouse NOV/CCN3 ELISA Kit |
| 产品描述 | For quantitative detection of mouse NOV in , cell lysates |
| 免疫原 | NSO, Q22-I354 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <5pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | NOV |
| 基因全名 | nephroblastoma overexpressed |
| Uniprot ID | Q64299 |
| 指标别名 | ccn 3 | CCN family member 3 | CCN3 | IGFBP9 | NOVH | IBP-9 | IGF-binding protein 9 | IGFBP9 | IGFBP-9 | nephroblastoma overexpressed | NovH | P48745 | Protein NOV homolog |
| 背景资料 | NOV (nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0834-CAP | 96-well plate precoated with anti- mouse NOV antibody | 1 |
| EK0834-ST | Lyophilized recombinant mouse NOV standard | 10ng/tube×2 |
| EK0834-DA | Biotinylated anti- mouse NOV antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 84 | 277 | 999 | 77 | 292 | 970 |
| Standard deviation | 3.86 | 14.12 | 49.95 | 4.62 | 18.98 | 49.47 |
| CV(%) | 4.6% | 5.1% | 5% | 6% | 6.5% | 5.1% |
Mouse NOV/CCN3 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.148 | 0.209 | 0.275 | 0.372 | 0.569 | 1.018 | 1.668 | 2.264 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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