Rat TNFSF11/RANKL ELISA Kit

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货号：EK1559

反应性：Rat

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EK1559-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK1559
产品名称	Rat TNFSF11/RANKL ELISA Kit
产品描述	For quantitative detection of rat TNFSF11 in , cell lysates
免疫原	E.coli, F141-D318
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	15.6pg/ml-1000pg/ml
基因名称	TNFSF11
基因全名	tumor necrosis factor (ligand) superfamily, member 11
Uniprot ID	Q9ESE2
指标别名	CD254 \| O14788 \| ODF \| OPGL \| RANKL \| TRANCE \| OPTB2 \| Osteoprotegerin ligand \| RANKL \| sOdf \| TNF11 \| TNFSF11
背景资料	Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. The rat gene is mapped to 15q11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
显色时间	15-20分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1559-CAP	96-well plate precoated with anti- rat TNFSF11 antibody	1
EK1559-ST	Lyophilized recombinant rat TNFSF11 standard	10ng/tube×2
EK1559-DA	Biotinylated anti- rat TNFSF11 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Rat TNFSF11/RANKL ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 15-20分钟)

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.087	0.145	0.202	0.315	0.522	0.909	1.581	2.347

*典型数据仅供参考。用户需要自己建立标准曲线。

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