Mouse CD70/TNFSF7/cd27L ELISA Kit

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货号：EK1310

反应性：Mouse

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK1310-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK1310
产品名称	Mouse CD70 ELISA Kit
产品描述	For quantitative detection of mouse CD70 in , cell lysates
免疫原	NSO, S45-P195
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	31.2pg/ml-2000pg/ml
基因名称	CD70
基因全名	CD70 molecule
Uniprot ID	O55237
指标别名	CD 27L\|CD 70\|CD27 L\|CD27 LG\|CD27 ligand\|CD27-L\|CD27L\|CD27LG\|CD70\|CD70 antigen\|CD70 molecule\|CD70_HUMAN\|Ki 24 antigen\|Ki24 antigen\|Surface antigen CD70\|TNFSF 7\|TNFSF7\|Tumor necrosis factor (ligand) superfamily, member 7\|Tumor necrosis factor ligand superfamily member
背景资料	CD70(Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.
显色时间	15-20分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1310-CAP	96-well plate precoated with anti- mouse CD70 antibody	1
EK1310-ST	Lyophilized recombinant mouse CD70 standard	10ng/tube×2
EK1310-DA	Biotinylated anti- mouse CD70 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Mouse CD70 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 15-20分钟)

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.001	0.053	0.124	0.252	0.615	1.209	1.932	2.175

*典型数据仅供参考。用户需要自己建立标准曲线。

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